or placebo o relative to sham instrumented control hearts at top left panel, top right and min bottom left panel mean lvdp of hearts from to min of reperfusion are shown in the bottom right panel tetrazolium celexa by vbulletin intitle view profile chloride stained heart slices showed that hearts treated with csil at , and celexa by vbulletin intitle aprovel avapro avalide irbesartan view profile min had similar injury as that of the sham operated hearts � , � celexa by vbulletin intitle view profile and � , and � respectively p � ns the infarct size of hearts celexa by vbulletin intitle view profile treated with csil even at min of global ischemia was � of the ventricles this was significantly smaller than its corresponding control p , whereas hearts celexa by vbulletin intitle view profile treated with control iggl at , and min of global ischemia were � , � celexa by vbulletin intitle view profile and � respectively fig , left panel the corresponding nitroblue tetrazolium chloride stained heart celexa by vbulletin intitle view profile slices are shown in fig , right panel another parameter of myocardial injury celexa by vbulletin intitle view profile that was determined was mitochondrial size although mitochondrial swelling is a hallmark of celexa by vbulletin intitle view profile ischemic injury, irreversible injury cannot be directly extrapolated from just observation of mitochondrial celexa by vbulletin intitle view profile size nevertheless, in view of the myocardial functional and his tochemical evidences, mitochondrial size assessment from transmission electron micrographs add additional support for myocardial preservation in csil treated hearts, relative to iggl or placebo treated hearts figure shows the comparison of fig mean infarct sizes of rat hearts treated with csil or iggl or placebo at and min of global ischemia left panel celexa by vbulletin intitle view profile the corresponding nitroblue tetrazolium chloride stained mid slices of rat hearts treated with csil or iggl at , and min of global ischemia minimal injury was seen in and min csil treated hearts, but injury was evident in the min celexa by vbulletin intitle view profile csil treated heart slice injury is evident in all heart slices treated with iggl right panel, bottom two rows �i i normal cut csil csil celexa by vbulletin intitle view profile csejo igsl tg& iflsi placebo v fig mean mitochondrial size of normal, csil, celexa by vbulletin intitle view profile iggl or placebo treated hearts treatment was as indicated in the text mitochondrial celexa by vbulletin intitle view profile size of normal hearts, csil treatment at and min of global ischemia, celexa by vbulletin intitle view profile as well as iggl treated hearts at , and min of global ischemia and celexa by vbulletin intitle view profile with placebo no difference in mitochondrial size was observed between normal myocardium � celexa by vbulletin intitle view profile mean number of pixels � sem and myocardium treated at and min celexa by vbulletin intitle view profile of global ischemia � , � , � and � respectively p = ns however, mitochondria of hearts treated with iggl at , and min of global ischemia or placebo � , � , � and � respectively were larger than mitochondria of csil treated hearts p these studies showed that myocardial viability preservation is not celexa by vbulletin intitle view profile restricted to embryonic cardiocytes in cultures adult hearts are also amenable to structural celexa by vbulletin intitle view profile and functional preservation, following cell membrane lesion sealing in a timedependent manner during ischemia this method of cell membrane lesion sealing has also been reported to preserve the integrity of vascular endothelium with antiactinimmunoliposomes a question that remains concerning the utility of csil is whether immunoliposomes can retain the protective functions celexa by vbulletin intitle view profile in the presence of plasma proteins in vivo, since experiments have demonstrated that cells in culture and adult hearts perfused with nonprotein oxygenated buffer were prevented from undergoing myocardial necrosis, following cell membrane lesion sealing intervention with cytoskeletal antigen specific immunoliposomes to demonstrate that cell membrane lesion sealing also occur in celexa by vbulletin intitle view profile vivo, rabbits with experimental myocardial infarction were used in this study, rabbits celexa by vbulletin intitle view profile were injected with antimyosin csil, plain liposomes or saline at the time of left circumflex coronary artery occlusion by intracoronary infusion the occlusion was kept for celexa by vbulletin intitle view profile min followed by hrs of reperfusion the hearts were excised, sliced into � celexa by vbulletin intitle view profile slices parallel to the short axis and stained with nitroblue tetrazolium chloride the infarct was approximately to of the infarcts of the control plain liposome celexa by vbulletin intitle view profile or saline treated rabbit hearts subsequently, comparison to iggliposome treated hearts with acute myocardial infarction demonstrated that the csil treatment resulted in significantly smaller infarct size, celexa by vbulletin intitle view profile as was observed in comparison to plain liposome or saline treated hearts thus, cytoskeletalantigen specific immunoliposomes, consisting of antimyosin or antiactinimmunoliposomes, were demonstrated to be celexa by vbulletin intitle view profile able to preserve cell viability and integrity its potential utility in the cardiovascular celexa by vbulletin intitle view profile system would be enhanced once its efficacy following intravenous delivery has been celexa by vbulletin intitle view profile demonstrated however, the study of asahi et al showed that intravenous delivery of celexa by vbulletin intitle view profile the antiactin immunoliposomes enabled preservation of the integrity of the endothelial cells of celexa by vbulletin intitle view profile the cerebral vessels csil as targeted gene or drug delivery due to the proposed mechanism of cell membrane lesion sealing, we also proposed that if celexa by vbulletin intitle view profile drugs or gene constructs were to be included in the immunoliposomes such as csils, then these drugs or gene constructs should be delivered directly into the cytoplasm fig this route should bypass the endocytic route of drugs or celexa by vbulletin intitle view profile gene construct delivery, thereby reducing destruction of the delivered cargo by the lysosomal enzymes, after formation of endolysosomes using silver grains as model fig transmission electron celexa by vbulletin intitle view profile micrographs of embryonic cardiocyte treated with silver grains impregnated csil left and plain liposome impregnated with silver grains right � = jim drugs, we demonstrated celexa by vbulletin intitle view profile that these drugs can be delivered directly into the cytoplasm of hypoxic cardiocytes celexa by vbulletin intitle view profile treated with silver grains loaded csils figure left shows a transmission electron micrograph celexa by vbulletin intitle view profile of a cardiocyte treated with silver grains impregnated csils silver grains in groups of concentration at about nm were observed however, in cells treated with silver grains impregnated plain liposomes, very few cells were viable of one such celexa by vbulletin intitle view profile cell detected by transmission electron microscopy, the silver grains were observed in the celexa by vbulletin intitle view profile extracellular space [fig right] fig diagrammatic representation of delivery of intraliposomally entrapped celexa by vbulletin intitle view profile genetic construct or drugs directly into the cytoplasm of target cell nmuin ftivoih wiiit mi ��� when the silver grains were replaced with genetic constructs, pgl celexa by vbulletin intitle view profile and psv gal vectors, hypoxic cardiocytes treated with csil impregnated with either vectors showed luciferase activity or bacterial jgalactosidase activity the successful transfection of the celexa by vbulletin intitle view profile hypoxic cardiocytes with pgl, a vector for firefly luciferase enzyme � � fig celexa by vbulletin intitle view profile relative light units of luciferase activity of cardiocytes treated with various preparations and controls in csils is shown in fig as relative light units rlus rlus were determined by the use of a luminometer as can be seen, only hypoxic cardiocytes treated with pglcsils showed increased rlus significantly above normal cells with treatment with no vectors similarly, normoxic cardiocytes treated with pglcsil, hypoxic cardiocytes and normoxic cardiocytes treated with plain liposomes, or with only vectors, showed no significant gene transcription and expression when hypoxic cardiocytes were treated with csil with entrapped psvgal vectors, almost all cells in the field of view under light microscopy exhibited bacterial �galactosidase enzyme activity, following reaction with xgal bromo chloroindolylbetadgalactopyranoside, nm mgcl,mm iqfetcn h, mm kfecn in phosphate buffered saline ph celexa by vbulletin intitle view profile [fig a] when this mode of gene expression was compared with transfection of celexa by vbulletin intitle view profile psvjgal vector with cationic liposomes, cationic liposome transfection according to the manufacturers protocol resulted in transfection of only a few cells per field of view [fig b], in this micrograph, two cells with intense ��galactosidase activity were observed quantitation of the number of cells in the field of view that was celexa by vbulletin intitle view profile successfully trans fected with psvjgal vector in csil, cationic liposome, iggliposomes, plain celexa by vbulletin intitle view profile liposome and vector alone are shown in fig c only csil and cationic celexa by vbulletin intitle view profile transfection showed gene expression csiltransfection or csilfection was more than times more efficient in transfecting cells than cationic liposomes although the intensity of gene expression was celexa by vbulletin intitle view profile low with csilfection, using the initial vector concentration of xg of vectors celexa by vbulletin intitle view profile in mg lipids in ml, when the vector concentration risperdal uses off label was increased to � celexa by vbulletin intitle view profile zg, also in mg lipids in ml, the intensity of gene transfection was increased [fig d], this study showed that approximately x xg ��� � m t� !