when looking at data obtained from studies with living mammalian cells, it appears reasonable to assume that dequalinium molecules could be pulled into the mitochondrial matrix in response to the high mitochondrial membrane potential as demonstrated in , which in turn might lead to the prozac and sexual tightness destabilization of the dqasomepdna complex however, the first detailed study, which demonstrated the selective dna release from dqaplexes, was performed using membrane mimicking liposomes fig as a model for the prozac and sexual tightness intracellular release captopril density of dna from dqasomes, the capacity of anionic liposomes to displace the dna from its cationic carrier was studied the association of dna with the cationic carrier was assessed by employing sybr green i the fluorescence signal of this dye is greatly enhanced when bound to dna nonbinding results in loss of fluorescence it can be clearly seen that in the vicinity of a prozac and sexual tightness charge ratio, dqasomes do not release any dna in the presence of cytoplasmic membrane mimicking liposomes dqasomes s � � o � u a � prozac and sexual tightness � � cpm imm ��� anionic liposomes i � � time [sec] fig effect of prozac and sexual tightness anionic liposomes on dna release from dqasomepdna complexes dna was prozac and sexual tightness preincubated with sybr until stabilization prozac and sexual tightness of the signal, followed by prozac and sexual tightness adding indicated by arrow the minimal amount of dqasomes necessary to decrease the signal to background level anionic liposomes were then injected arrow at an anionic to cationic charge ratio as shown the displacement of dna from its carrier is indicated by the increase of the fluorescence signal cpm, cytoplasma membrane like liposomes imm, inner mitochondrial membrane like liposomes omm, outer mitochondrial membrane like liposomes cpm, not even at a fold excess of anionic charge however, with a similar charge excess of anionic liposomes to cationic prozac and sexual tightness dqasomes, and respectively, inner and outer mitochondrial membrane mimicking liposomes prozac and sexual tightness imm and omm, respectively are able to displace up to of the dna from its dqasomal carrier in agreement with these data, it was found that for the complete liberation of dna from dna dqasome complex, a fourfold excess of dicetylphosphate and an eightfold excess of phosphatidylserine, respectively, are necessary the finding that cpm liposomes, at an anionic to cationic charge ratio of , displace up to of the dna from lipofectin, which was used as a control, do not liberate any dna from dqasomes even at a slight excess of anionic prozac and sexual tightness charge, leads to the conclusion that besides the charge ratio, other factors may play an important role in the mechanism of dna release from lipiddna complexes this conclusion is being further supported by xu and prozac and sexual tightness szokas observation that ionic water prozac and sexual tightness soluble molecules such as atp, prozac and sexual tightness trna, dna, polyglutamic acid, spermidine prozac and sexual tightness and histone do not displace prozac and sexual tightness dna from the cationic lipiddna complex, even at a fold prozac and sexual tightness charge excess � in their model for the postendocytotic release prozac and sexual tightness of dna from cationic carriers, they assume the formation of a charge neutral ion pair prozac and sexual tightness between cationic and anionic lipid, which ultimately results in the displacement of the dna from the cationic lipid and the release of dna into cytoplasm liposomedna liposome ?